1. Remember to be empty (be sure to insert the ultrasonic horn into the sample before turning it on)
2, the horn (ultrasound probe) water depth: 1.5Cm or so, the liquid level is preferably 30mm or more, the probe should be centered, do not stick. Ultrasonic waves are vertical longitudinal waves. If they are inserted too deeply, it is not easy to form convection and affect the efficiency of crushing.
3, ultrasonic parameter setting: set the key instrument operating parameters (specific settings see instructions or call us), for the temperature sensitive requirements of the sample (such as bacteria) generally outside the use of ice bath, the actual temperature must be less than 25 degrees, protein Nucleic acids certainly do not degenerate.
1) Time: The ultrasonic time should not exceed 5 seconds at a time. The gap time should be greater than or equal to the ultrasonic time to facilitate heat dissipation. The time setting should be based on the principle that the ultrasonic time is short and the number of ultrasound times is large, which can prolong the life of the ultrasonic machine and the probe.
2) Ultrasonic power: It should not be too large, so as to avoid splashing or foaming of the sample. For example, the sample capacity is less than 10ml, the power should be less than 200w, the 2mm ultrasonic probe should be used, and the horn selector switch on the back of the panel should be switched to the corresponding gear; The power of 200ml sample capacity is 200-400w, 6mm ultrasonic probe is used, and the horn behind the panel is driven to the corresponding gear; the capacity of the sample above 200ml is between 300-600w, 10mm ultrasonic probe is used, and the rear of the panel is used. The horn is hit to the corresponding gear; (the small probe power of 2MM is strictly prohibited to exceed 350W)
3) Container selection: How many samples are selected for the larger beaker, which is also advantageous for convection in the ultrasound to improve the efficiency of the crushing. For example; a 20ML throughput is best used with a 20ML beaker.
Such as 100ml E. coli sample setting parameters: Ultrasound 5 seconds / gap 5 seconds The number of times 70 times (total time is 10 minutes). Power 300W (for reference only)
The amount of 500ML or so, the power is about 500W-800W
4. If the sample is placed in a 1.5ml EP tube, be sure to fix the EP tube to prevent the liquid level from falling after the ice bath melts.
5, daily maintenance: scrub the probe with alcohol or use ultrasonic water for ultrasound after use!
2, the horn (ultrasound probe) water depth: 1.5Cm or so, the liquid level is preferably 30mm or more, the probe should be centered, do not stick. Ultrasonic waves are vertical longitudinal waves. If they are inserted too deeply, it is not easy to form convection and affect the efficiency of crushing.
3, ultrasonic parameter setting: set the key instrument operating parameters (specific settings see instructions or call us), for the temperature sensitive requirements of the sample (such as bacteria) generally outside the use of ice bath, the actual temperature must be less than 25 degrees, protein Nucleic acids certainly do not degenerate.
1) Time: The ultrasonic time should not exceed 5 seconds at a time. The gap time should be greater than or equal to the ultrasonic time to facilitate heat dissipation. The time setting should be based on the principle that the ultrasonic time is short and the number of ultrasound times is large, which can prolong the life of the ultrasonic machine and the probe.
2) Ultrasonic power: It should not be too large, so as to avoid splashing or foaming of the sample. For example, the sample capacity is less than 10ml, the power should be less than 200w, the 2mm ultrasonic probe should be used, and the horn selector switch on the back of the panel should be switched to the corresponding gear; The power of 200ml sample capacity is 200-400w, 6mm ultrasonic probe is used, and the horn behind the panel is driven to the corresponding gear; the capacity of the sample above 200ml is between 300-600w, 10mm ultrasonic probe is used, and the rear of the panel is used. The horn is hit to the corresponding gear; (the small probe power of 2MM is strictly prohibited to exceed 350W)
3) Container selection: How many samples are selected for the larger beaker, which is also advantageous for convection in the ultrasound to improve the efficiency of the crushing. For example; a 20ML throughput is best used with a 20ML beaker.
Such as 100ml E. coli sample setting parameters: Ultrasound 5 seconds / gap 5 seconds The number of times 70 times (total time is 10 minutes). Power 300W (for reference only)
The amount of 500ML or so, the power is about 500W-800W
4. If the sample is placed in a 1.5ml EP tube, be sure to fix the EP tube to prevent the liquid level from falling after the ice bath melts.
5, daily maintenance: scrub the probe with alcohol or use ultrasonic water for ultrasound after use!
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