Model NO.: T-Bil
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*15 mL
Origin: Jinan, Shandong
Model NO.: T-Bil
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*15 mL
Origin: Jinan, Shandong
Total Bilirubin (T-Bil) assay kit is applied to measure the concentration of T-Bil in human serum or plasma.Testing Principle
In the presence of surfactant and accelerating agents, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.
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Product Features
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1. Linear range of T-Bil level: [1, 684] μmol/L
Linear correlation coefficient ∣r∣≥0.990Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.10 Â
3. Sensitivity
The change in absorbance (ΔmA) ≥ 0.30 while testing the specific T-Bil sample of concentration of 83.1 μmol/L.4. Accuracy
Relative deviation is within ±10%.5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤5.0%.Technical Parameters
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| Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
| Main wavelength | 450 nm | Secondary wavelength | 546 nm (Optional) |
| Method |
End-point method (dual reagents) |
Reaction direction | Negative |
| Sample | 8 μL | R1 | 240 μL |
| R2 | 60 μL | Reaction time | 300 s |
Packing Specifications
R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)
Storage and Stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
 Total Bilirubin (T-Bil) assay kit is applied to measure the concentration of T-Bil in human serum or plasma.
Testing Principle
In the presence of surfactant and accelerating agents, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.
  Â
Product Features
Â
1. Linear range of T-Bil level: [1, 684] μmol/L
Linear correlation coefficient ∣r∣≥0.990Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.10 Â
3. Sensitivity
The change in absorbance (ΔmA) ≥ 0.30 while testing the specific T-Bil sample of concentration of 83.1 μmol/L.4. Accuracy
Relative deviation is within ±10%.5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤5.0%.Technical Parameters
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| Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
| Main wavelength | 450 nm | Secondary wavelength | 546 nm (Optional) |
| Method |
End-point method (dual reagents) |
Reaction direction | Negative |
| Sample | 8 μL | R1 | 240 μL |
| R2 | 60 μL | Reaction time | 300 s |
Packing Specifications
R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)
Storage and Stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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