Contamination and rapid quantitative detection of aflatoxin in bean paste - accurate quantification in -8min, authoritative verification by the Grain Bureau

First, the contamination of aflatoxin B1 in bean paste

Food safety has become a big concern for the whole people. Douban is an essential spice for most dishes, and its safety issues have received more and more attention. The Pixian County watercress is a local famous product in Sichuan Province and is one of the favorite spices for people. Broad bean is one of the main raw materials of the watercress of Pixian County, and its quality and safety directly affect the product quality of the finished watercress. Broad beans are stored and transported under humid conditions and are susceptible to mildew. The mycotoxins present in broad beans are mainly aflatoxin (AFT), Fusarium toxin, followed by variegated toxin and aflatoxin A. Among them, aflatoxin B1 is the most toxic and carcinogenic, and its toxicity is much higher than that of hydride, arsenide and organic pesticides.

At normal cooking temperatures, aflatoxin is rarely destroyed and cannot be completely destroyed even under high temperature heating at 200 Â°C. In China, there have been several poisoning events caused by aflatoxin in the watercress. The aflatoxin B1 (AFB1) in the exported watercress has become a technical barrier to export.

Second, the aflatoxin B1 national limit standard in bean paste

Worldwide, based on the toxicity of these mycotoxins, the levels of mycotoxins in food and zoo raw materials are controlled.

The limit for aflatoxins in EU countries is: AFB1 is limited to 2Î¼g/kg; the total amount of AF is limited to 4Î¼g/kg.

Third, the necessity of aflatoxin B1 detection

As the main raw material for the production of traditional fermented products, it is necessary to strengthen the detection of aflatoxin in broad beans, and it is also the raw material that must be kept in production. Existing national standards have specified a method for detecting aflatoxin in some products, but the method is cumbersome, and it is difficult for enterprise inspectors to master. Moreover, in the aflatoxin detection method, pretreatment of the sample is very important. Therefore, the establishment of a stable, reliable and rapid detection method for aflatoxin B1 in broad beans is very important for improving the quality of watercress.

4. Rapid quantitative detection of aflatoxin B1 in Chinese herbal medicines in Shanghai Feizheng - accurate quantification in -8min

Based on the leading fluorescence quantitative FPOCT technology platform, Shanghai Festo Biotechnology is the first to introduce aflatoxin B1 fluorescence quantitative rapid detection system, including aflatoxin detector and aflatoxin fluorescence quantitative rapid test strip, which can be quickly and accurately quantified in 8min. The detection of the residual content of aflatoxin B1 in the bean paste, the sample pretreatment is simple, the detection operation is simple, the result is accurate and reliable, and can be printed on site, the accuracy is in accordance with the detection result of the HPLC method, and is suitable for various bean paste processing enterprises, the first Three-party testing agencies and government regulatory agencies.

Rapid quantitative detection of aflatoxin B1 in Shanghai Feishu biomedicine - accurate quantification in -8min

4.1. Aflatoxin B1 Fluorescence Quantitative Rapid Detection System Performance

ïµ detection sensitivity: 0.5Î¼g / kg;

ïµQuantitative linear range: 1.0Î¼g/kg - 75.0Î¼g/kg;

ïµ sample pretreatment time: 7min;

ïµ Detection time: 8min;

ïµAccuracy: recovery rate is 80%-125%;

ïµ specificity: no cross-reactivity with other mycotoxins at a concentration of 1000 Î¼g/kg;

Aflatoxin B1 Fluorescence Quantitative Rapid Detection System

4.2. Sample preparation process

1. Crushing (smashing and processing of bean paste sample, weighing);

2. Oscillation extraction (5min);

3. Centrifugation (2min);

4.3. Detection operation process

1. Dilution;

2. Sample addition reaction (8 min);

3, reading, print test report;

Aspergillus flavus detection operation process in peanut

4.4. Results interpretation and output

Reading with a portable aflatoxin B1 detector makes the test results more accurate and objective, avoiding human error.

Shanghai fly test biomyxin toxin detector

The test result will be presented on the liquid crystal display of the fluorescence reader. At the same time, the paper test report can be obtained by pressing the print button. In addition, after the WIFI data upload function of the instrument is turned on, the relevant data information will be automatically uploaded to the â€œFood Safety Traceability Managementâ€. Cloud platform for easy traceability and quality management.

4.5. Shanghai fly test biomycotoxin series fluorescence quantitative test strip product advantages

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