Dear, are you still worried about scratching experiments?
After 6 hours of entanglement, staying up until 2 in the morning, just to look at him under the microscope. The amount of the gap between them is narrowing.
No matter how sleepy, you have to open your eyes and kneel under the microscope to distinguish whether the vision you saw this time was the one you observed last time. However, he always finds him not to find.
I hope that there will be such a device that can return to the original position of the first treatment group after the control group is taken. Moreover, it can automatically shoot while cultivating, and there is no fear of losing focus.
Finally , he finally appeared. "HoloMonitor M4 Laser Holographic Cell Imaging and Analysis System" can help you solve all problems, eliminate various intermediate links and directly receive results.
Wound healing is a natural process of regeneration of human skin and epidermal tissue after trauma. Normally, the skin's surface (outermost layer) and dermis (internal or deep) are in a balanced state to form a barrier to protect the wound. When the eschar is broken, the normal wound healing process begins quickly.
A simple and cost-effective in vitro test for cell migration/tumor invasion in cell scratch assays. The principle of this method is that when the cells grow to a single layer state, a blank area is artificially created on the fused monolayer cells, becoming a "scratch." The cells at the edge of the scratch will gradually enter the blank area to cause the "scratches" to heal.
The traditional experimental method is:
After the cells are covered by a single layer, they are scratched on the surface of the cell layer with a pipette tip.
Problems and defects:
1. Artificially manufactured scratches are difficult to ensure consistency and poor repeatability.
2. It is easy to cause dead cells to accumulate on both sides of the scratch, forming a hill-like area that hinders the healing of normal cells.
M4 uses 635nm low-intensity near-infrared light that does not harm the cells, uses coherent laser illumination and diffraction principle to clearly record the information of each part of the cell on the CCD, and reconstructs the real cells on the computer by software algorithm. 3D stereoscopic form. The software automatically focuses in real time, and 4 groups of samples are simultaneously observed.
The M4 can be built in a CO2 incubator for shooting while training. Just set the total duration, time interval, shooting field of view, click start, no need for any personnel to assist in the whole process.
Together with IBIDI we can provide a more accurate scratch test: there is no cell death in the scratched area and the spacing is controllable.
The output is accurate and diverse, covering the need to publish articles
- Each image can be output and the resulting Video Illustrates how the cells migrate to healing.
- Automated kinetics monitors the closing time of the gap and the area covered by the cells.
- Cell front-end movement speed, migration distance per cell, migration direction, movement distance and movement rate. In addition, there are cell parameters for each cell, including surface irregularities, eccentricity, cell area, volume, thickness, etc.
- Migration path of wound edge cells
- A scatter plot of the direction of cell migration, depicting the direction of migration of the cells, and the migration trajectory
- Export data is presented in excel
- Data accuracy and repeatability
- The left picture is the experimental data statistics of M4 combined with IBIDI; the right picture is the experimental data statistics made by the traditional method. Wound healing analysis has been widely used to mimic the in vivo migration of cells under normal or pathological conditions. HoloMonitor Wound Healing Assay not only reveals the level of cell population in wound healing, but also monitors the condition of each individual cell. The application of this unlabeled real-time 3D tracking imaging technique to wound healing experiments is bound to lead to a revolution in cell migration research.
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