Breaking cells and extracting high-purity, highly active proteins or peptides is a key goal for researchers and biotech professionals. Among various methods, ultrasonic disruption is widely used due to its simplicity. The process involves using ultrasonic waves in a liquid to create cavitation, which breaks down cell structures. However, this method has several drawbacks: it can be costly, generates significant heat, and may produce free radicals that inactivate sensitive biological molecules. Additionally, the effectiveness of sonication varies depending on the type of microorganism—bacilli are generally easier to break than cocci, and Gram-negative bacteria are more susceptible than Gram-positive ones. Yeast, in particular, is more resistant to this technique.
As research advances and demand for high-activity biomolecules increases, traditional ultrasonic methods often fall short. This has led to the development of alternative techniques such as high-pressure cell disruption. Unlike ultrasonic methods, high-pressure systems force the sample through a narrow chamber at high velocity, causing shear forces, collisions, and cavitation that effectively rupture cells. Pressures exceeding 100 MPa are considered ultra-high, and under these conditions, the structural integrity of microbial cells is compromised, leading to cell lysis and inactivation.
A notable advancement in this field is the low-temperature ultra-high pressure continuous flow cell disruptor. This technology addresses the thermal challenges associated with traditional high-pressure methods by maintaining the process at 4–6°C using a circulating water bath. The cooling system absorbs the heat generated during cell disruption, preserving the activity of delicate proteins and peptides. Through extensive design optimization, the device achieves an ideal balance between shear, collision, and cavitation forces, ensuring efficient cell lysis without compromising molecular integrity.
In a comparative study, both an ultrasonic cell disruptor and a low-temperature ultra-high pressure continuous flow cell disruptor were used to lyse the same amount of *E. coli*. The results showed that the polypeptide activity obtained via the new system was 155 U/mg, compared to 110 U/mg from the ultrasonic method—a 40% improvement. This highlights the superior performance of the low-temperature system in maintaining protein activity.
This innovation represents a significant step forward in cell disruption technology, offering a more effective and reliable solution for researchers aiming to extract high-quality biomolecules.
The main effects and production process of Amino Acid chelate: improve bioavailability: after the metal ions in amino acid chelate are combined with amino acid, its absorption and utilization in human body or plants and animals are greatly improved, improve stability: amino acid chelate is more stable in and out of the body and does not easily react with other components, thus maintaining the activity of minerals.
Promote plant growth: In agriculture, amino acid chelate can be used as trace element fertilizer to improve the absorption of trace elements by plants and enhance their disease resistance and growth rate.
High purity amino acids and metal salts are selected as raw materials. Common amino acids include glycine, lysine, etc. Metal salts include zinc sulfate, magnesium sulfate, etc. The amino acid solution is mixed with the metal salt solution in a certain proportion and the reaction is carried out under suitable pH and temperature conditions. This process is usually carried out in a stirred reactor to ensure a homogeneous reaction.
The quality of the produced amino acid chelate is tested to ensure that its purity and stability meet the requirements. Finally, the product is packaged to prevent moisture and contamination.
Amino acid chelates are widely used in human and animal nutritional supplements and plant fertilizers because of their high efficiency and safety.
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