Tips and solutions for using pure water

In the rapid propagation of tissue culture, the reagent used in the laboratory is high-purity water. A laboratory with an annual output of 3 million seedlings requires more than 15 kg of high-quality pure water per day. If distilled water is used to prepare the medium, the cost is too high, wasting energy and labor. The purpose of using pure water in the tissue culture laboratory is to clearly understand and grasp the composition of the medium, to ensure a high rooting rate and differentiation rate, and to prevent precipitation of the mother liquor in a short period of time. The pure water prepared by the ion exchange water purifier can fully meet the requirements of the tissue culture laboratory, and the cost is low, the equipment is simple, the water output is large, and the daily use can be made.

The following describes the problems, solutions and tips used in the preparation of pure water. Common problems and solutions 1. The resin is exposed to oily substances. The contaminated oil substances easily block the pores of the resin, and the active groups of the micropores are blocked, so that the active groups cannot be exchanged. In particular, when the tap water of the newly installed water pipe is used as the water seal instead of the raw material belt, the oily tap water quickly saturates the resin, and the oil passes through the pure water device, and the effluent water quality deteriorates. With this pure water-containing medium, the rooting rate is almost zero. When this happens, the resin can be soaked in an alkaline saline solution to 40--45 ° C for 2 hours, and then do it again according to the new resin pretreatment method. It is also treated with a nonionic surfactant soaking treatment.

2. The poisoned anion resin of the anion resin is repeatedly used, contaminated by degradation products of the organic compound and the cation resin during the aging process, the exchange capacity is decreased, and the elution time is prolonged during regeneration, and an anion activation treatment is required. Activation method: use 70% ethanol solution containing 3% salt, soak the anion resin for 2 hours, stir from time to time, and then do it according to the new resin pretreatment method, and then regenerate it according to the routine, which can shorten the regeneration and rinsing time.

3. The yin and yang column confusion is due to the fact that the yang 1# and yin 5#, yin 3# and yang 4# exchange columns have the same water outlet and appearance, and are prone to occur in Yang 1# and Yin 5#, Yin 3# and Yang 4# Interchange position. The regenerated resin which is regenerated by alkali treatment or the regenerated anion resin which is treated with an acid has no ion exchange ability. The anion resin, which was placed in the wrong position at the beginning, retains the color of the original new resin after two years of use, and can be called a resin without work. At this time, the quality of the pure water produced is very unstable, the exchange capacity is small, and the rooting condition of the tissue culture material is unstable.

The solution is to take out the resin of 6 columns, put it in the saturated brine, stir it, the yang resin sinks underneath, the anion resin floats on it, there is a blank salt layer in the middle, and the upper layer of yin resin is removed with a net bucket. The cation and cation resins are completely separated. It can not be judged according to the color of the resin. The anion resin which does not work is lighter in color and more like a cation resin.

4. When the water purifier is deactivated for a long time, the water in the column is replaced every 3 days. If the water does not change for more than 20 consecutive days, the resin will undergo oxidation reaction, and impurities will be released one after another. After releasing 40L of water in the front stage, the water is not qualified. The water produced at this time will affect the hardness of the medium. At this time, only the resin can be taken out completely, and the new resin is pretreated.

5. Horizontal water production of water purifier When the water purifier is placed horizontally, the resin in the column is in a discrete suspension state, which cannot function as filtration and adsorption. The effluent water quality is the same as that of tap water, and may even have resin poisoning emissions, water quality ratio. Tap water is even worse.

6. Regenerative rinsing is not enough according to the instruction manual of the water purifier. When the anion resin is regenerated, it can be washed to pH 8~9. In the tissue culture laboratory, the single salt mother liquor used to prepare the zinc sulfate, manganese sulfate, magnesium sulfate, etc., which is commonly used in the tissue culture chamber, causes precipitation. The reaction with zinc sulfate is quick. The anion resin regeneration rinse of the tissue culture chamber is rinsed to pH 7.

7. The ambient temperature of the water purifier causes the gum to deteriorate when the water purifier is close to the heat source or the sun is irradiated for a long time, and the effluent is alkaline. At this time, the water produced does not precipitate a large amount of element mother liquor or zinc sulfate, and has no effect on the tissue culture seedlings, but the rooting time is postponed for 2 to 3 days.

Practical skills 1. Two sets of pure water are used to divide the water into two purity levels. The pure water produced by the pure water purifier has high purity and is used to prepare a large amount of element and hormone mother liquor. After the total water production reaches 200L, the water required for the production medium is degraded, and after regenerating 300L, the resin is regenerated. Processing, enter the next cycle. In this way, according to the characteristics of the water in the tissue culture room, the water purifier can be fully utilized to reduce the cost.

2. Water quality testing for general chemical analysis with pure water, resistance is greater than 0.5MI2. Cm is qualified. Take the resistance is greater than 0.5MI2. The water detection of cm, the anion and cation are not detected. The tissue culture laboratory uses 0.1 to 0.3MI2. Cm pure water can be. It is more reliable to use the conductivity meter to detect water quality. However, since the water purity instrument attached to the water purifier is susceptible to moisture and short circuit, it is often faulty. We actually use one by one to pay attention to the protection of the ecological environment, and do not cause environmental damage. Destructive. "In the forest park, areas with good ecological functions and normal functions of ecological functions should be adopted to adopt more strict protection measures to prevent new human-induced damage and degradation. For areas with degraded ecological environment and functional degradation, it is necessary to develop ecology early. Restoration and reconstruction. Improve the ecological environment management monitoring mechanism of protected areas, form a scaled ecological function protection system, and realize the overall and all-round protection of the ecological functions of forest parks.

Gynaecology Products

Gynaecology Products,Cervical Scraper,Disposable Endometrial Cannula,Gynaecological Brush

Luck Medical Consumables Co.,LIMITED , https://www.luckmedical.com

Posted on