Virus extraction and processing

Generally 3,000 live crickets can take 3 grams of wet poison, processed into dry powder is 1 gram. After taking wolfberry fruit, it can be taken again every 15 to 20 days after taking poison (feeding temperature should be above 28°C). Pregnancy can still produce Aberdeen after taking poison, but it will affect the survival rate of Aberdeen. The best time for taking drugs is from the end of spring to the end of the fall of the current year. The wolfberry fruit production in October is reduced. In October, it is basically no detoxification. It enters winter pods and cannot be used for poisoning. Need to use the tools used are: electric pulse detector, 10 ml small beaker, vacuum pump, dryer, brown glass bottles, refrigerators, latex gloves and so on. First, take poison. From May to October, it is the best season for scorpion activity, foraging, growth, and development. It is also the season when scorpions have the highest amount of poison and the best quality. The best temperature for extracting poison is better than 25°C. The lice used for poisoning should reach the age of 6 to 7 years after reaching sexual maturity. At this time, the detoxification amount of the lice is large, and the growth and reproduction of the lice will not be affected after the application of the poison. Before taking the poison, brush the razor clams into the large plastic basin from the rearing tank, put a damp clean towel on the pelvic floor, and spray a small amount of physiological saline to fix the dust on the surface of the ladles so that they do not fly. The operation steps of the electric pulse instrument are as follows: turn on the instrument switch, adjust the voltage to 8 to 12 volts, adjust the frequency to 128 Hz, and insert the wires of the electrode soft pen and the wires of the harvesting rack into the corresponding jacks. Turn on the power supply; soak the electrode soft tip on the rack with saline solution, grasp the end of both ends of the tweezers with the left hand, and use the electrode to continuously touch the front chelation of the tweezers to stimulate the tweezers to excrete the venom and collect them in a beaker (beaker bottom requires Put the ice cubes and change them every 2 hours to ensure the quality of the active enzyme for scorpion venom.) Put the dumplings of the venom into plastic pots; place the scorpion venoms in a vacuum dryer to dry them. deal with. Second, the dryness of venom. The commonly used drying method is vacuum drying. Under low pressure conditions, the moisture in the venom can quickly evaporate. The vacuum drying apparatus includes a vacuum drier, a condenser tube, and a vacuum pump. The piston at the top of the dryer is connected to the condensing tube. The other end of the condensing tube is connected to the suction bottle, the dryer and the vacuum pump. Desiccant and venom samples were placed in the desiccator. Before use, apply a small amount of petrolatum around the piston of the dryer and check the entire device for leaks. When in use, first put the venom and desiccant into the petri dish, then place the petri dish in the desiccator, start the vacuum pump, and pump to the lid to stop. Then close the piston first and then close the vacuum pump. After the scorpion venom is dry, the piston should be slowly unscrewed to prevent the air from escaping the toxic dry powder. The dry powder should also be taken out under clean conditions and immediately put into a brown glass bottle and sealed. Third, the scorpion virus extraction process. The fresh venom was removed by electric stimulation. The container containing narcotics was kept at 0°C-4°C in ice cubes, and centrifuged at 2500-4000 rpm for 30 minutes to separate impurities such as soil and calcium. Pure maggots were obtained. , Quick-cooling vacuum drying to obtain white crystal powder, packaging in different specifications of the bottle, the product is stored in a dry low temperature and light conditions. The entire process can be summarized as follows: 2 hours for poisoning, 20 to 30 minutes for purification at 0°C to 4°C, 5 minutes or 2 hours for quick freezing at 20°C to 25°C, and 4 to 6 hours for drying at -19°C to -14°C, -5 °C ~ 20 °C freeze-dried vacuum drying into dry powder.

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