Over the past few years, we have accumulated some experience in the study and practice of plant propagation and cultivation, and we share with you. Only if we do more exchanges and learn from each other can we promote the sustainable development of technology. There are deficiencies, I hope my friends have a lot of criticism and advice, thank you!
Rosemary is a perennial dwarf shrub or indica woody plant of the Lamiaceae family. The original Mediterranean coast has green, needle-shaped leaves with a strong, fresh grassy aroma. The flowers are blue, purple, pink and white. Exudes rich aromas and is widely used in perfumes, baths, cosmetics, soaps, air fresheners, food seasonings, and more. It has the pharmacological effects of promoting blood circulation, reducing gallbladder blood pressure, preserving antibacterial activity, and anti-cancer effects. The plant can also be used for water and soil conservation, landscaping, home potting, and has the function of repelling mosquitoes. It is an economic plant with high comprehensive development and utilization value. Rosemary leaves can be used to extract essential oils and antioxidants. Many developed countries, especially the United States, Japan, and France, have listed them as important economic plants for development and have invested a lot of money in research and development. In recent years, many research institutes in China have carried out research on seed introduction, cultivation, essential oils, antioxidants, processing and refining, and achieved certain results. However, due to the single species, poor quality, and shortage of seedlings, it has limited the Promotion and application. This post has conducted several experiments and studies on rosemary cutting propagation and other methods. The content is now written below. Friends are welcome to discuss.
The cutting materials were potted plants of the standard rosemary Labiatae introduced from the United States and the cultivars Blue lagoon and Upright of the plants introduced from the Netherlands. The matrix uses perlite, peat soil, yellow soil, coarse sand. Rooting accelerators use IBA and indole acid, indole acetic acid or naphthalene acetic acid as single or compound ingredients.
The establishment of ear-sprouting aids and the harvesting of cuttings were selected from the plants introduced and planted, and explants were used to induce the production of tissue-cultured seedlings (examples of tissue culture induction were further detailed in subsequent articles). After the pots grow to 30-40cm as the parent plant to establish a scion. The mother plants of the ear-sprout were cultivated, and the mothers of the ear were bred to sprout a large number of lateral branches for harvesting. The 3-5 cm shoots and the 7-10 cm semi-lignified young shoots were cut from the mother plants of the ear for cuttings. The cuttings should be cut. Place a person in a container filled with clean water and cut the base leaf of the cutting.
The cuttings were compared with IBA 0 or 50 or 100 or 500 mg/L concentrations and different hormone concentrations of LX2 rooting powder to treat shoots and tender shoots; perlite: Hesha (1:2), peat: perlite (2:1), peat soil: river sand (2:1), peat soil "Yellow earth soil (2:1) different substrate tests. Abiatae, Blue lagoon, and Upright different varieties, different seasons young shoots nursery seedlings. All to be inserted At 0.5cm from the base of the louse, the IBA diluent is soaked in the LX2 (1000IPQL) rooting powder or 1-3cm at the base for 0.5h. Each batch is treated with 500 cells and divided into 5 groups (5 replicates). The strains were statistically average rooted at 30 days after cuttings.
The cutting method is to cut the treated cuttings according to the requirements of the experimental design. The cuttings were sterilized with 0.1g/L potassium permanganate solution for 3-5 minutes before cutting, and then washed with clean water. The substrate was sterilized with 35% formaldehyde 20ml/L solution for 15 days. When the cuttings are first punched in the matrix, the depth is 1/3-1/2 of the cuttings. After inserting, the matrix around the cuttings is compacted, soaked with water and moistened with a plastic film.
After the cuttings, check the slotting machine frequently to remove dead plants and rotten leaves in a timely manner. The 7d interpolating bed should keep the air humidity above 85%, and then gradually reduce; the second day spraying fungicide, and then the weekly spraying fungicide, 1g/L Potassium nitrate and monobasic potassium dihydrogen phosphate are mixed once to prevent infection by cuttings and promote rooting and growth. Part of the membrane is opened regularly every day for ventilation and ventilation. 10d after cutting roots can be transplanted into a nutrition bag or flower pot cultivation, leaching 2g/L of nitrogen, phosphorus, potassium mixture once a week, the other according to conventional nursery nursery method management.
Effect of Matrix on Rooting Rate of Cuttings
Cuttings use photosynthetic nutrients or leaves contained in the plant's vegetative organs to supplement the nutrients provided by the photosynthesis to supply hairy roots (before hair roots). At this time, the organic matter present in the matrix easily picks up pathogenic bacteria, causing germs to invade, causing the cuttings to rot. At the same time, the matrix contains a certain amount of fertilizer is conducive to the growth of the cuttings after rooting. In addition, the matrix used for cutting requires good moisture retention, good drainage, and fixed physical structure of the cuttings. From the results of the experiment, the rooting rate of various substrates was relatively high, reaching more than 95%, and there was no significant difference, but the roots had different degrees of sturdiness. In contrast, Perlite: Hesha (1:2) substrate roots had an average rooting rate of 98%, and the roots were thick, many and long; the average rooting rate of peat soils: Huang Xintu (2:1) was also 95. %, but it's fine, and it's small and short. Therefore, the specific operation can select the cutting matrix according to the actual situation.
Effects of Cutting Season on Cutting Rooting Rate
The temperature of the cutting environment changes with the season in a year, and its level affects the physiological activity of the cutting and the growth of the bacteria, thereby affecting the cutting rate. According to the experimental results, the cutting rooting rates of cuttings in different seasons of the year are different, with the highest rooting rates in spring (April, 15-26 degrees) and winter (December, 12-24 degrees), reaching 98% and 94 respectively. %, followed by autumn (October, 2-30 degrees), with a rooting rate of 88%, with no significant difference between them; while summer (July, 26-33 degrees) has the lowest rooting rate, only 55%, and others There was a significant difference in seasons. The test results showed that cuttings and rooting of rosemary cuttings were suitable in Guangzhou, spring and winter.
Effect of Exogenous Hormones on Rooting
Exogenous hormone treatment of cuttings can affect the rooting, select the appropriate hormone and its concentration, can increase the rooting rate and the survival rate of transplanting. The degree of lignification of cuttings also affects the rooting rate of cuttings. The experimental results concluded that the rooting rate of the shoots treated with IBA concentration of 0.50 mg/L and LX2 was the highest, 93%, 98%, 95%, respectively, with no significant difference between them; the IBA concentration was 500 mg The rooting rate of the /L treatment was the lowest, only 82%, and showed significant differences with other treatments; the rooting rate of the treatment with 100 mg/L concentration was 92%, showing a significant difference from the concentration of 50, 500 mg/L; The rooting rates of shoots at the concentration of 50, 100 mg/L and LX2 were the highest, 95%, 98%, and 94%, respectively, and there was no significant difference between them. The rooting rate of the treatment with 500 mg/L IBA was the lowest, only 86%, and showed significant difference with other treatments. The rooting rate of IBA blank concentration was 92%, which was significantly different from the concentration of 100,500 mg/L; The results also showed that the higher the degree of lignification of cuttings, the higher the rooting rate of cuttings increased with the increase of IBA concentration, while it decreased immediately when the concentration rose to 500 mg/L.
Comparison of rooting rates of different varieties of cuttings
From a series of experimental results, it can be seen that the rooting rate of different varieties of cuttings is different. The rooting rate of the spikes of Labiatae is the highest, reaching 94%, and is significantly greater than the other two varieties. The roots are thick, and they are long and many. The second is Blue Lagoon, which has a rooting rate of 87% and is also significantly larger than Upright. The rooting rate of the latter is also 80%. The roots of both are thick and long but less.
In order to provide a more mature and more comprehensive cutting technology for the promotion and application of production, on the basis of the above, a large-scale cutting production pilot plant that simulates the production of field conditions before the cutting production is also carried out, and is transplanted for cultivation. From the results, the rooting rate and the survival rate of transplanting for each batch of cuttings were relatively high, all at 89% and 92% and 93%, and the averages were about 91% and 90%, respectively, so that they could be mass-produced. development of.
Rosemary can be used in tissue culture seedling shoots and tender shoots cutting propagation, cutting rooting fast, rooting rate is high, 7-13d after cutting rooting, 30d after seedling transplanting. Cuttings and seedlings experiments showed that rosemary is a rooting type cutting plant. For the cutting of this type of plant, the key effect is to control the environmental conditions of the cutting according to its biological characteristics. In the cutting technique, it is generally accepted that hormone treatment can increase the rooting rate of cuttings, but for the cutting-prone rooting plants, the hormone treatment effect is not obvious, on the contrary, an excessively high hormone concentration will inhibit the rooting. The degree of lignification of cutting substrates and shoots had little effect on the rooting rate of the cuttings, but it affected the rooting time and root growth and development, while the variety, hormone concentration and cutting season had a greater influence on the rooting rate of cuttings. . Proper illumination and a permeable, moist matrix facilitates rooting of the cuttings.
Hope friends further discuss and improve! Thank you!