Bottle planting
With a 750 g, 4 cm diameter bottle, a wide mouth or can bottle can also be used.
Mix the bottled main ingredients and accessories, etc., mix the sugar, dissolve the sugar in the water, gradually add the ingredients and mix thoroughly, add 140-160 kilograms of water per 100 kilograms of dry material, and grasp a small amount of water droplets in the fingers. The next is appropriate. Use unarmed or bottling machine for dispensing, requiring moderate elasticity, until it is 1 cm away from the bottle mouth, flattened, the center is punched with cypress to the bottom of the bottle, the bottle mouth and bottle wall dirt are washed, and the bottle mouth is wrapped with kraft paper. .
Sterilization and inoculation were carried out at normal pressure at 100°C for 8-12 hours, autoclave at 121°C and hold for 1.5 hours. After cooling, bacteria are introduced under aseptic conditions. Then put into the training room, control the temperature 25 °C, indoor relative humidity controlled at 70% or less. The culture chamber is kept dark to facilitate mycelial growth. After 20 days of bacterial culture, when the mycelium grows to 1/2 bottle, the sealing paper can be removed and moved to the physical chamber. At this time, the temperature is controlled at 25°C-28°C, and the relative humidity is increased to 90%-95%. The air is kept fresh and the diffuse light is enhanced. The cap is continuously growing until the white growth circle around it disappears, which means that it stops growing and expanding, but it can still be thickened. The fruiting body forms a tube of bacteria and ejects red-brown spores.
Bag planting
Similar to the bottle planting process, but using polyethylene or PVC plastic bags, about 10 cm away from the bag mouth, close the bag mouth, sets of plastic collars, plus tampon. Sterilize and inoculate culture under sterile conditions. When the mycelium grows to about 1/2 of the bag, the tampon and collar are pulled out, exposing the material surface and letting it out. Be careful not to open the bag too early to prevent the mycelium from drying out and affecting the fruiting body.
Wood cultivation
Choose hardwood species such as eucalyptus, eucalyptus, birch, etc. The tree age is 7-10 years and the diameter is 6-15 cm.
Cut down the cuttings After the leaves fall to the bud before the leap year, do not damage the bark when cutting. Saw into sections before inoculation, soaking in water for 12-15 hours, increasing the moisture content to facilitate the fungus.
Perforated inoculation with a belt punch installed in the inoculation hammer drilling diameter of about 9.6-10 mm, then use the inoculation gun into the bacteria species, and then use the bark cover to close the hole, inoculation election sunny day, so that the side hole , inoculation, edge closure points.
The bacteria were stacked in a “well†shape, about 1 meter high. The bottom was filled with stones or waste wood. The temperature in the stack was controlled to be above 20°C and below 28°C. When the temperature was high at noon on sunny days, the film should be ventilated and cooled. Each week, the wood is turned up and down, inside and outside, and promotes even bacteria. After about 30 days, the inoculation hole and the bark were covered with hyphae and germination was over.
The buried soil, bacteria, and fruiting bodies are well-selected for drainage. There is a bed of water or shade under the shade. The trampoline is 1.2 meters wide and 20 cm deep. The wood was discharged horizontally in a bed of bacteria, with a 5 cm section of wood. The wood gap was filled with a fine loam soil without impurities, and then the soil was covered with 2 cm of soil. The shelter is 60 centimeters high from the bed of the fungus. Covered with thin grass curtains, the cover is covered with a film to prevent direct light and heavy rain. During this time, reduce the water supply and keep the bacteria for about 30 days. The yellow-white hyphae on the cross-sections at the end of the section of trees form the stage of the fruiting body, increasing the humidity of the space, spraying water 1-2 times in the sunny day to keep the soil moist. In early July, sub-entities began one after another. The second year of May-July was booming, and there were still fruiting bodies in the third year.
Harvest processing
When the white growth circle around the cap disappears and the whole cap is reddish-brown and brown powdery spores appear, the fruit body can be removed. It usually takes about 50-60 days from inoculation to harvesting. After harvesting, it is dried on the bamboo screen and dried at low temperature. To collect spore powder can be collected using the bagging method or the shelf closing method, and then the fruiting bodies are extracted.
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