The medical biotechnology research center of the Ministry of Health and the scientific staff of the Guangzhou City Center for Disease Control have collaborated to establish a new technology for the rapid detection of coronaviruses using quantitative real-time PCR. This technology is part of the results of the Ministry of Public Health and the Ministry of Science and Technology's â€œAccident and Atypical Pneumonia Prevention and Control Science and Technology Actionâ€ and is expected to provide reliable experimental diagnostic indicators for the early rapid diagnosis of atypical pneumonia. He Yunxiu, professor of the Medical and Biological Engineering Technology Research Center of the Ministry of Health, said on the 17th to the reporter of Xinhua News Agency that the use of this new technology, from taking patient samples to reporting results, can be completed within 2 hours, enabling the realization of viral genes. Accurate quantitative detection can reflect the occurrence and development of the disease. PCR is an in vitro gene replication technology that can amplify genes to millions or more times in hours or tens of minutes, making genes easy to detect. He Yunxi said that the coronavirus nucleic acid was detected in the four patient cultures and mouthwashes provided by the Guangzhou City Center for Disease Control, and none of the three confirmed SARS patients were found. In addition, no coronavirus nucleic acid was detected in the gargles of 30 healthy individuals. After analyzing the results of the sequencing of the gene amplification fragment of 4 positive samples of coronavirus, the homology of the coronavirus gene sequences published in Canada and the United States was compared. 100%.
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