<p> Commonly used reagents and methods of use I. Diagnostic paper (1) Bacitracin paper (0.04U/tablet): Inhibition zone >10mm is sensitive. Quality control strain: Group D streptococcus negative, group A streptococcus positive.
(2) SMZ paper (1.25 μg/tablet, 23.75 μg/tablet): Sensitive zone is sensitive. Quality control strain: group D streptococcus positive, group A streptococci negative.
(3) Neomycin paper (5.0μg/tablet): The inhibition zone ≥16mm is sensitive. Quality control strain: Staphylococcus epidermidis positive, staphylococcus aureus negative.
(4) O129 paper, Optochin paper: See Biochemical Test Medium.
Second, the diagnosis of serum
1. Salmonella diagnostic serum (1) AF group O multivalent diagnosis serum.
(2) Specific O group factor diagnosis serum: commonly used O2, O4, O7, O9, O10 diagnostic serum.
(3) Specific H factor diagnostic serum commonly used Ha, Hb, Hc, Hd, Hgm, Hi, Hf, Vi factor diagnostic serum.
2. Shigella genus 4 kinds of multivalent serum of Shigella genus: Shigella dysenteriae I and II serum, Shigella flexneri multivalent serum and typing serum (type 1 to 6), Song Shiga Bacterial diagnosis of serum, Shigella baumannii multivalent serum and typing serum.
3. Pathogenic Escherichia coli diagnosis of serum enteropathogenic Escherichia coli (EPEC) diagnostic serum, enterotoxin-producing Escherichia coli (ETEC) diagnostic serum, enteroinvasive Escherichia coli (EIEC) diagnostic serum , Enterohemorrhagic Escherichia coli (EHEC) diagnosed serum O157: H 7.
4. Vibrio cholerae O1, O139 mixed multivalent diagnosis serum and rice leaf, Ogawa, Hikoshima O139 typing serum
5. Neisseria meningitidis multivalent serum and group serum
6. Streptococcus classification diagnosis serum
7. Streptococcus pneumoniae diagnosis serum
8. Yersinia diagnosis of serum three, commonly used staining solution
1. Gram staining solution (1) Crystal violet solution A solution: crystal violet 20 g, 95% ethanol 20 ml; B solution: ammonium oxalate 0.8 g, distilled water 80 ml.
Mix liquid A and liquid B 24 hours before dyeing, filter and put into reagent bottle for use.
(2) Iodine solution: 1 g of iodine, 2 g of potassium iodide, and 300 ml of distilled water.
Iodine is mixed with potassium iodide and ground, a few milliliters of distilled water is added, gradually dissolved, and then ground, and a small amount of distilled water is added to the iodine, and potassium iodide is completely dissolved. After the zui, make up the amount of water. It is also possible to completely dissolve potassium iodide with a small amount of distilled water, and then add iodine tablets. After completely dissolved, water is added to 300 ml.
(3) Decolorization solution: 95% ethanol (4) Re-staining solution: 2.5 g of sand yellow, 100 ml of 95% ethanol as a stock solution, take 10 ml of the stock solution, and add 90 ml of distilled water as the application liquid.
2. Anti-acid staining solution (1) Alkaline complex red staining solution: 1 Cannes carbolic acid red solution: alkaline reddish ethanol saturated solution 10ml, 5% carbolic acid solution 90ml. 2 Decolorization solution: 3 ml of concentrated hydrochloric acid, 97 ml of 95% ethanol. 3 counterstaining solution (Luffel blue solution): 30 ml of a saturated solution of methylene blue ethanol, 0.1 ml of a 100 g/L potassium hydroxide solution, and 100 ml of distilled water.
(2) Amine O-Rhodamine B staining solution: 1 Rhodamine B solution: Rhodamine B 0.1 g plus distilled water 100 ml. 21g/L gold amine O solution: 0.1g of gold amine O solution and 95ml of distilled water, then add 5ml of pure carbolic acid and mix. 33% hydrochloric acid ethanol. 4 Dilute the blue liquid: 100 ml of rifle blue water, add 90 ml of distilled water, and mix.
3. Flagella dyeing solution (modified Ryu method)
Solution A: 10 ml of 5% carbolic acid, 2 g of citric acid, 10 ml of saturated potassium aluminum sulfate solution; B solution: crystal violet ethanol saturated solution. Apply 10 parts of liquid A solution, 1 part of liquid B, mix and store at room temperature for use.
4. Heterochromatic particle dyeing solution A solution: toluidine blue 0.15g, malachite green 2g / L, 95% ethanol 2.0ml, distilled water 100ml. Liquid B: 2 g of iodine, 3 g of potassium iodide, and 300 ml of distilled water.
First add potassium iodide to a little distilled water (about 2ml), shake well, until completely dissolved, then add iodine, so that after complete dissolution, add distilled water to 300ml.
5. Capsule staining solution (1) Indian ink or 50g/L melanin aqueous solution (2) 5g/L aniline blue aqueous solution
(2) SMZ paper (1.25 μg/tablet, 23.75 μg/tablet): Sensitive zone is sensitive. Quality control strain: group D streptococcus positive, group A streptococci negative.
(3) Neomycin paper (5.0μg/tablet): The inhibition zone ≥16mm is sensitive. Quality control strain: Staphylococcus epidermidis positive, staphylococcus aureus negative.
(4) O129 paper, Optochin paper: See Biochemical Test Medium.
Second, the diagnosis of serum
1. Salmonella diagnostic serum (1) AF group O multivalent diagnosis serum.
(2) Specific O group factor diagnosis serum: commonly used O2, O4, O7, O9, O10 diagnostic serum.
(3) Specific H factor diagnostic serum commonly used Ha, Hb, Hc, Hd, Hgm, Hi, Hf, Vi factor diagnostic serum.
2. Shigella genus 4 kinds of multivalent serum of Shigella genus: Shigella dysenteriae I and II serum, Shigella flexneri multivalent serum and typing serum (type 1 to 6), Song Shiga Bacterial diagnosis of serum, Shigella baumannii multivalent serum and typing serum.
3. Pathogenic Escherichia coli diagnosis of serum enteropathogenic Escherichia coli (EPEC) diagnostic serum, enterotoxin-producing Escherichia coli (ETEC) diagnostic serum, enteroinvasive Escherichia coli (EIEC) diagnostic serum , Enterohemorrhagic Escherichia coli (EHEC) diagnosed serum O157: H 7.
4. Vibrio cholerae O1, O139 mixed multivalent diagnosis serum and rice leaf, Ogawa, Hikoshima O139 typing serum
5. Neisseria meningitidis multivalent serum and group serum
6. Streptococcus classification diagnosis serum
7. Streptococcus pneumoniae diagnosis serum
8. Yersinia diagnosis of serum three, commonly used staining solution
1. Gram staining solution (1) Crystal violet solution A solution: crystal violet 20 g, 95% ethanol 20 ml; B solution: ammonium oxalate 0.8 g, distilled water 80 ml.
Mix liquid A and liquid B 24 hours before dyeing, filter and put into reagent bottle for use.
(2) Iodine solution: 1 g of iodine, 2 g of potassium iodide, and 300 ml of distilled water.
Iodine is mixed with potassium iodide and ground, a few milliliters of distilled water is added, gradually dissolved, and then ground, and a small amount of distilled water is added to the iodine, and potassium iodide is completely dissolved. After the zui, make up the amount of water. It is also possible to completely dissolve potassium iodide with a small amount of distilled water, and then add iodine tablets. After completely dissolved, water is added to 300 ml.
(3) Decolorization solution: 95% ethanol (4) Re-staining solution: 2.5 g of sand yellow, 100 ml of 95% ethanol as a stock solution, take 10 ml of the stock solution, and add 90 ml of distilled water as the application liquid.
2. Anti-acid staining solution (1) Alkaline complex red staining solution: 1 Cannes carbolic acid red solution: alkaline reddish ethanol saturated solution 10ml, 5% carbolic acid solution 90ml. 2 Decolorization solution: 3 ml of concentrated hydrochloric acid, 97 ml of 95% ethanol. 3 counterstaining solution (Luffel blue solution): 30 ml of a saturated solution of methylene blue ethanol, 0.1 ml of a 100 g/L potassium hydroxide solution, and 100 ml of distilled water.
(2) Amine O-Rhodamine B staining solution: 1 Rhodamine B solution: Rhodamine B 0.1 g plus distilled water 100 ml. 21g/L gold amine O solution: 0.1g of gold amine O solution and 95ml of distilled water, then add 5ml of pure carbolic acid and mix. 33% hydrochloric acid ethanol. 4 Dilute the blue liquid: 100 ml of rifle blue water, add 90 ml of distilled water, and mix.
3. Flagella dyeing solution (modified Ryu method)
Solution A: 10 ml of 5% carbolic acid, 2 g of citric acid, 10 ml of saturated potassium aluminum sulfate solution; B solution: crystal violet ethanol saturated solution. Apply 10 parts of liquid A solution, 1 part of liquid B, mix and store at room temperature for use.
4. Heterochromatic particle dyeing solution A solution: toluidine blue 0.15g, malachite green 2g / L, 95% ethanol 2.0ml, distilled water 100ml. Liquid B: 2 g of iodine, 3 g of potassium iodide, and 300 ml of distilled water.
First add potassium iodide to a little distilled water (about 2ml), shake well, until completely dissolved, then add iodine, so that after complete dissolution, add distilled water to 300ml.
5. Capsule staining solution (1) Indian ink or 50g/L melanin aqueous solution (2) 5g/L aniline blue aqueous solution
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