Pertussis ELISA kit
Germany IBL China exclusive agent "Shenzhen Kerunda Biological Engineering Co., Ltd." dedicated to serve you
Intended use: detection of humoral immune status after vaccination
The difference between acute and recent infection diagnosis
Epidemiological research
Advantages: complete typical and single antigenase-free test range
All pertussis test steps are the same
Antigen: FHA+PT+LPS, Tohama strain
Qualitative or quantitative analysis
Serum and plasma sample evaluation
High analytical sensitivity, broad linear test range
High specificity >95%
Single antigen detection, corrected according to international standard WHO06/140
Pertussis enzyme test data
Incubation time: 60/30/20min room temperature
Sample size: 5ul serum, plasma (1:101)
Ready-use reagent
How to operate the serological diagnosis of whooping cough
Pertussis is a persistent cough caused by a respiratory infection that can cause the disease from newborn to elderly. Bordetella pertussis produces a range of biologically active molecules such as pertussis toxin (PT), filamentous hemagglutinin (FHA) and various lipopolysaccharides (LPS). These compounds will appear during the onset or during the immunization process. Cell vaccines certified in Europe include PT and FHA, and more than 90% of the responses are directed against PT and FHA immunogens.
Serological diagnosis is judged by detecting a significant increase in antibody titer (paired serum) or a single high antibody titer (single serum)
The classic pertussis enzyme test we offer is based on a mixture of preferred antigens that can be used for dual serological testing.
product | Numbering |
Pertussis IgA | RE56131 |
Pertussis IgG | RE56141 |
Pertussis IgM | RE56151 |
Laboratory diagnosis of acute whooping cough infection is a problem. The experiment is based on a mixed antigen that cannot distinguish between pertussis B and pertussis B and cannot be standardized. Pertussis B is the main cause of whooping cough. Parapertussis B causes only mild symptoms, PT is expressed by pertussis B, and FHA is associated with other B. basidioides or microorganisms. The antigen cross-reacts, so the specificity is low.
Single antigen detection for FHA and PT has been developed and normalized to WHO reference materials. Detection of anti-PT antibodies is recommended for the diagnosis of acute pertussis B single serum samples, anti-FHA antibody detection can be used for transmission, immunogenic or non-inferior vaccine research.
Several reference centers in Europe have suggested double-threshold values ​​for anti-PT IgG:
>100 IU/ml has controversy over acute pertussis B infection
product | Numbering |
Pertussis PTIgG | RE56061 |
Pertussis FHA IgG | RE56091 |
References: Guiso, N., G. Berbers, NK Fry, Q. He, Riffelmann M., Wirsing von König CH, What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories. Eur. J Clin Microbiol Infect Dis.30.307-312 (2011)
Exclusive distributor in China: Shenzhen Kerunda Bioengineering Co., Ltd.
Company address : 6th Floor, No. 10, Yanshan Road, Shekou, Nanshan District, Shenzhen. Zip code : 518067
Contact number (8 lines) Fax : +86 755 26814431
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