Rapid detection of Cronox sinensis in food by PCR method with amplified internal standard

K. sinensis (formerly known as Enterobacter sakazakii) is a conditional pathogen of Enterobacteriaceae that can cause death in infants and young children. The current study found that K. sinensis is widely found in a variety of foods, such as dairy products, meat products, vegetables, fruits, cereals and so on. In some cases, the mortality caused by Crohn's disease can reach 40% to 80%. Therefore, strengthening the detection of Cronobacter sinensis is very important to ensure food safety. At present, the traditional culture of C. sinensis - biochemical identification method is complex, long detection period, low sensitivity, far from meeting the requirements of modern rapid detection. The PCR-based method is fast, sensitive, specific, and suitable for high-throughput operation, and becomes a common method for rapid detection. Studies have shown that the use of PCR detection methods for the actual detection of pathogenic bacteria in food samples will be affected by environmental factors and food physicochemical properties, including food matrix complex, medium composition and DNA extraction reagent residues and other reasons will affect the detection accuracy Sex, causing false negative results. Studies have shown that the addition of amplified internal standards plays an important role in ensuring the reliability of detection results in the PCR detection method system of food samples. An effective and applicable rapid detection method for foodborne pathogens must include only false negatives. The resulting amplified internal standard.

Rapid detection of Cronox sinensis in food by PCR method with amplified internal standard

Rapid detection of Cronox sinensis in food by PCR method with amplified internal standard

Rapid detection of Cronox sinensis in food by PCR method with amplified internal standard

Rapid detection of Cronox sinensis in food by PCR method with amplified internal standard

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