First, the collection of venomous lice In the case of anger, due to the defense or attack instinct, the venom will be discharged from the sac. The collection of narcotics is based on this principle. The commonly used methods for collecting scorpion venom include the trimming, artificial stimulation and electrokinetic methods. (1) Tail-cutting method: One hand holds tweezers on both sides of the fifth quarter of the abdomen of tweezers, cuts the tail end of the tweezers with scissors in one hand, immerses it in saline after crushing, leaches toxic parts, and concentrates toxic liquids. Into the container, and then made into dry powder, placed at -5 °C and stored for use. This kind of poisoning method can only collect poison once for each scorpion, and the scorpion after poisoning reduces the medicinal function and economic value, and the damage to the scorpion is too large, so usually this method is not generally adopted. (b) artificial stimulation method: usually use a tweezers clip a scorpion chelonian limb, lifted hanging in the container for a while, the majority of tweezers will emit the tail venom, but there are a few scorpions do not drain the venom, no venom can be used The chopsticks gently hit the head and chest or front abdomen of the scorpion, stimulating the tail thorn detoxification of the scorpion. (3) Electrostimulation: Electrostimulation is the collection of scorpion venom by electronic pulse-pumping instruments. This method has a large amount of toxins, high work efficiency, and one person's operation. Many times in the year to collect the poison without damaging the dumplings, it is a more advanced and scientific method for collecting poisons in China. 1. Equipment and equipment: electronic poisoning equipment, a wooden poisoning table (available two drawers desk instead) one. A 5 mm thick glass plate with an area similar to that of the bench was placed on the work table to place the poison extraction equipment on the glass plate. One 60-ml beaker, one hair dryer, one watering can, one pair of latex gloves, a little talcum powder, one stainless steel needle, one sieve for 20 mesh (the inner wall of the sieve should be affixed with a wide transparent tape, making it smooth to prevent lice. Escape), specifications are: height 15 cm, length 60 cm, width 50 cm. 2. Toxic method: Wash and sterilise the beaker, drain the water, weigh it and put it on the glass plate of the workbench. Place a stainless steel needle in the beaker. The length of the needle should be slightly higher than the cup. Put the dumplings ready for poisoning into the sieve for 2 hours. After allowing the dumplings to test each other's bodies and attachments, use a hair dryer to blow off the dust from the carcasses, and then spray a small amount of water onto the carcasses with a watering can. The saline was used for poisoning. Workers should wear clean white work clothes and work caps during poison collection, rub a few talcum powders with hands and wear latex gloves. Then turn on the power of the electronic pulse instrument. Insert the two animal tweezers plugs with splices and place the foot switch on the floor near the right foot. Both hands picked up two scorpions, one on each side of the fifth quarter of the abdomen, and the other scorpion caught the first quarter of the scorpion's tail so that the scorpions faced the belly downwards. The stinger faced the beaker and moved closer to the beaker. Inside, the foot switch is powered on for 1~2 seconds, the lice will automatically discharge the venom, and immediately lift the foot and disconnect the power. If the venom of the stinger tip hangs venom, immediately try it on the steel needle inside the beaker. Detoxify the hanging venom. 3. Note: 1 In the process of drug collection, we must pay strict attention to hygiene and ensure the purity and quality of the poison. 2 There are individual scorpions that do not detoxify during the first pass. They must be turned on again. However, the power-on time must be maintained for 1 to 2 seconds and must not exceed 2 seconds in order to avoid burning the instrument and damaging the scorpion. 3 The amount of detoxification of the dumplings varies with temperature and varies. When the temperature is high, the amount of detoxification is relatively high. When the temperature is low, the amount of detoxification is relatively small. When the temperature is lower than 20° C., the detoxification amount of the dumplings is quite small, and some are almost in stagnation. When the temperature is below 10°C, the dumplings stop detoxification. Therefore, if you want to collect poisons at room temperature, you must start collecting poison when the temperature in June is above 25°C. When the temperature drops gradually below 25°C in the month, it is better to stop collecting poisons. 4 Pre-pregnancy maternal pregnancies and fleas cannot be used for poisoning. The dumplings used for poisoning are mostly commercial and old. However, it is better to use larger males that do not use for breeding. Males, individuals, and large amounts of detoxification, can disperse 4 to 5 drops at the most. Second, the initial processing of scorpion venom scorpion venom liquid is easily degraded at room temperature, and can only be stored in the refrigerator for about half a month, and must be processed into a dry powder before it can be stored for a long time. The processing method is as follows: liquid scorpion venom is first frozen in the refrigerator, and then transferred into a vacuum drier after freezing, and an appropriate amount of calcium oxide is used as a desiccant at the bottom of the drier. The desiccant is covered with four layers of new gauze and placed on the gauze. With a virulent beaker, followed by pumping with a vacuum pump, during the pumping process, attention should be paid to observation. If a large amount of air bubbles are found on the surface of the venomous poison, it is necessary to stop for a while and then pump until it is basically dry and let it rest for 24 hours. The scorpion venom is thoroughly dried and becomes crystal grains of unequal size. This is the crude dry powder that has been processed for the first time. Scrape the dry poison powder into small bottles, dissolve the wax and seal it, label it, indicate the preparation date and weight of the scorpion venom powder, and put the opaque black paper on it. Store in a refrigerator at -5°C for 5 years without deterioration. .
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