First, the detection principle
The kit uses a competitive ELISA method to pre-coat the anti -nitroimidazole antigen on the microplate of the microplate . After detection, standard or sample solution is added, and enzyme-labeled antibody-nitroimidazole was coated nitroimidazole competitively added to the sample and the antigen binding nitroimidazole antibody, and then antigen-antibody complex is formed with an enzymatic label The substance was developed with TMB substrate; the reaction stop solution was added and detected under a 450 nm wavelength microplate reader, and the concentration of the nitroimidazole in the sample was inversely proportional to the intensity of the absorbed light.
Second, the scope of detection
This kit is a technology developed ELISA detection of drug residues in the product, compared to the instrumental analysis of the economy can be detected quickly nitroimidazoles honey.
Third, the cross-reaction rate
Cross-reaction rate with analogs:
Metronidazole (MNZ) Â ............................................. 100%
Dimethyl imidazole nitrate DMZ .......................................... 168%
Los Nitazoxanide (RNZ) Â ............................................. 112 %
................................................... 33% isopropyl nitazoxanide
Fourth, the kit consists of
1 plate , 96 holes / plate
6 bottles of standard solution ( 1ml / bottle) :
0 ppb , 0.05 ppb , 0.2 ppb , 0.8 ppb , 3.2 ppb , 12.8 ppb
Antibody working solution .............................. 7 ml
Enzyme label ........................... 12 ml
Substrate A ..............................7ml
Substrate B ..............................7ml
Stop solution ..............................7ml
20X concentrated washing solution .................. 40 ml
2X concentrated solution .................. 50 ml
Instructions .................................1 copy
Sample processing steps :
( A ) Honey ( sample dilution factor of 0.5 )
( 1 ) Take 3 g of honey, add 3 ml  0. 1 M  The carbonate buffer solution is shaken until the honey is completely dissolved;
(2) was added 9 ml of dichloromethane, shaken for 5 minutes at room temperature 4000 r / min, and centrifuged for 5 min;
(3) removing impurities upper layer, the lower layer was the organic phase to another 6 ml centrifuge tube was added 2 ml of methylene chloride, 2 ml 2M sodium hydroxide solution at room temperature for 4000 rev / min, and centrifuged for 5 min;
(4) Take 4 ml to the clear organic phase was dried under a laminar dissolution vessel, 50 ℃ water bath, blown dry with nitrogen;
(5) Working reconstituted with 0.5 ml of dried residue was dissolved, then hexane was added 1ml mixed for 30 seconds at room temperature at 3000 rpm / min or more, centrifuged for 5 minutes;
( 6 ) Remove the upper layer and remove 50 μl of the liquid for analysis.
Fiber Optic Cleaning Gun,Fiber Optic Cleaning Foam Swab,Swabs Foam Gun Swabs,Foam Swab Sterlied
Hunan Kangfutai Medical Devices Co., Ltd. , https://www.3kfut.com