How to reproduce daffodils

Commonly used ball, sowing and tissue culture breeding.

Taper propagation: Lateral buds within the bulb expand to form daughter bulbs. When the bulbs are dug out in the fall, the bulbs are propagated for branching, and the natural reproduction rate is 4 to 5 times. The rate of flowering of the main bulb is 100%, and the flowering rate of the lateral bulb is 80% to 90%. In order to increase the propagation coefficient, the daughter bulbs can be artificially induced. Using a sharp knife, the full bulb is cross-cut longitudinally from the bulb plate to the top by 3 to 4 knives at a depth approximately equal to the height of the bulb's Rongchangsheng/2, in order to impair the growth point of the shortened stem. After cutting, the bulbs were placed in clean dry sand to produce callus, which was then placed in a 21°C breeding box. The temperature was gradually increased to 30°C, relative humidity was 85%, and most of the bulbs were formed in about 3 months. Can be removed from the planting. Induced sub-bulbs are cultivated for 3 to 4 years as flowering bulbs.

Sowing propagation: sowing in mid-September. Sowing the earth with rot leaf soil, peat and coarse sand mixed soil, after disinfection, put pots to be sown. Fine management after sowing, the spring emergence, there is a leaf. In early summer, leaves and roots withered one after another to form dormant bulblets. Bulblets need to be cultivated for 4 to 5 years to become flowering bulbs.

Tissue culture propagation: bulbs or shoot tips, stem disks as explants. Wash the detergent first, and then disinfect it with 75% alcohol and 0.1% mercury for 30 minutes, then rinse it with sterile water 3 times. Inoculated on MS medium supplemented with 2,4-D2 mg/L and kinetin 0.1 mg/L and transferred to MS plus 6-benzylamino adenine 2 mg/L and naphthaleneacetic acid 1 mg/month after 2 months The medium was grown for about 15 days to form seedlings and then transferred to rooting medium of 1/2 MS plus 1 mg/L of butyric acid for 10 days. Rooted shoots were formed after 10 days, and flowering bulbs were grown after 3 years.

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